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Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: Cardiovascular Therapeutics

Article Title: The miR‐511‐3p/ATP2A2 Axis Is Required for Propofol‐Mediated Cardioprotection Against Hypoxia/Reoxygenation Injury

doi: 10.1155/cdr/7021184

Figure Lengend Snippet: Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Colorimetric quantitative analysis was performed with lactate dehydrogenase (LDH) assay kits (Abcam, United Kingdom) and malondialdehyde (MDA) detection kits (Elabscience, China).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture